Darcie L. Moore

Position title: Associate Professor, Department of Neuroscience

Email: darcie.moore@wisc.edu

Phone: (608) 265-7836

Darcie Moore headshot

Department:

Neuroscience

Education:

Ph.D. in Neuroscience, University of Miami School of Medicine, Miami, FL, USAPostdoc, ETH Zurich / University of Zurich, Zurich, Switzerland

Research Description:

The overarching focus of my research through the years has been regeneration of the adult central nervous system. There is limited regenerative capacity in the adult mammalian central nervous system (CNS). My lab studies this loss of regeneration in two different contexts:

Adult Neurogenesis, where neural stem cells (NSCs) make newborn neurons in the adult brain.

  • NSCs in the hippocampus generate newborn neurons throughout life in a process referred to as adult neurogenesis. Adult NSCs are primarily quiescent, in a reversible G0 state. Upon receiving a signal, quiescent NSCs (qNSCs) activate, entering the cell cycle to initiate population expansion, differentiation, maturation, and integration. During aging and disease, extrinsic and intrinsic factors drive adult hippocampal NSCs deeper into quiescence, reducing NSC quiescence exit, ultimately contributing to cognitive decline. My lab works to identify factors controlling NSC quiescence and quiescence exit to improve neurogenesis and ultimately identify targets to enhance cognitive function. We specifically have focused our efforts in the following areas:
    • Proteostasis
    • Translational control
    • Asymmetric inheritance of specific cellular cargoes during mitosis
    • Creation of imaging tools to identify qNSCs
    • How this translates to cancer

CNS Axon Growth and Regeneration following an injury.

  • Despite decades of study in rodent models, there is still no “cure” for CNS injuries such as spinal cord injury (SCI). The lack of human-specific and age-specific models of axon growth may be a crucial limiting factor in identifying translational targets for treatment of human SCI. Thus, we used direct reprogramming of human fibroblasts to neurons, bypassing pluripotency to maintain the age of the original cell, to create human neurons of different ages. We hypothesize that a species- and age-specific model will lead to the identification of axon growth regulators that could be targeted for development of more effective therapies for human SCI. Our research in this area has focused on:
    • The developmental loss of intrinsic axon growth ability in human neurons
    • Epigenetic regulation of axon growth and regeneration

Specifically we determine how changes during development and aging influence these aspects of regeneration.

To address these questions, the Moore lab uses cell biology, biochemistry, molecular biology, genetics, and computational approaches. We also specifically focus on using advanced live imaging technologies, including FLIP, FLIM, FRAP, photoactivation, 4D timelapse, in vivo cranial window imaging, and computer learning-based high-throughput imaging to address our scientific questions. If you can see it, you can believe it.

Research Key Words:

Neural Stem Cells, Aging, Axon Regeneration, Central Nervous System Regeneration, Asymmetry, Quiescence

Diversity Statement:

The diversity of people and opinions enriches our research and our world, and I am invested in supporting and promoting diversity and inclusion in our lab and the greater scientific community. I am committed to mentoring a diverse population: not only recruiting, but maintaining, and advancing their careers in STEM fields. I have acted as a mentor judge for both SACNAS (Society for Advancement of Chicanos/Hispanics and Native Americans in Science), and ABRCMS (The Annual Biomedical Research Conference for Minority Students) societies.

Link to Lab Website

Link to Publications