- Professor, Department of Pediatrics
- (608) 263-3579
Ph.D. Yokohama City University, Japan
Neuroendocrine Neurons in the Hypothalamus
Many types of neuroendocrine neurons in the hypothalamus release neuropeptides into the pituitary portal circulation in an oscillatory manner, which is essential for normal pituitary function. My research focuses on the study of one of these neurons--the gonadotropin releasing hormone (GnRH) neurosecretory system. The aims of current studies are: 1) to determine the mechanism of the onset of puberty, which requires an increase in GnRH pulse frequency; 2) to investigate the role of locally synthesized and released neuroestradiol in regulation of GnRH release; 3) to study the mechanism of ovarian steriod action on GnRH neurons, which modulate pulsatile GnRH release with different time domains, and 4) to generate GnRH neurons from stem cells and investigate the cellular mechanisms of pulsatile LHRH neurosecretion.
My research is being conducted on the rhesus monkey, as an important model for humans. I take a multidisciplinary approach to accomplishing the research aims, and use a variety of techniques including a microdialysis method to determine in vivo release of neuropeptides, neurotransmitters, and neurosteriods; Ca2+ imaging in GnRH neurons; identification of neuroendocrine neurons with immunocytochemistry and in situ hybridization; and assessment of quantitative neuronal gene expression and DNA methylation with various molecular biological approaches.
Kenealy, B.P., Keen, K.L., Garcia, J.P., Richter, D.J., and Terasawa, E. 2015 Prolonged infusion of estradiol benzoate into the stalk median eminence stimulates release of GnRH and kisspeptin in ovariectomized female rhesus macaques. Endocrinology 156: 1804-1814. PMCID: PMC4398774
Kenealy, B.P., Kapoor, A., Guerriero, K.A., Keen, K.L., Garcia, J.P., Kurian, J.R., Ziegler, T.E., and Terasawa, E. 2013 Neuroestradiol in the hypothalamus contributes to the regulation of gonadotropin releasing hormone release. J. Neurosci. 33: 19071-19085. PMCID: PMC3850033
Terasawa, E., Guerriero, K.A., and Plant, T.M. 2013 Kisspeptin and puberty in mammals. Adv Exp. Med. Biol. 784: 253-273. PMCID: PMC3996677
Kurian, J.R., and Terasawa, E. 2013 Epigenetic control of gonadotropin releasing hormone neurons. Front. Endocrinol. (Lausanne). 4: 61. PMCID: PMC3664328
Terasawa, E., and Kenealy, B.P. 2012 Rapid action of estradiol and GnRH neurons. Front. Neuroendocrinol. 33: 364-375. PMID: 22940545
Terasawa, E., Kurian, J.R., Keen, K.L., Shiel, N.A., Colman, R.J., and Capuano, S.V. 2012 Body weight impact on puberty: Effects of high calorie diet on puberty onset in female rhesus monkeys. Endocrinology 153:1696-1705. PMCID: PMC3320255
Fuenzalida, L.C., Keen, K.L., and Terasawa, E. 2011 Colocalization of FM1-43, Bassoon and GnRH-1: GnRH-1 release from cell bodies and their neuroprocesses. Endocrinology 152: 4310-4321,. PMCID: PMC3199012
Terasawa, E., Kurian J.R., Guerriero K.A., Kenealy B.P., Hutz, E.D., and Keen, K.L. 2010. Recent discoveries on the control of GnRH neurons in nonhuman primates. J. Neuroendocrinol. 22: 630-638. PMCID: PMC 2908205
Noel, S.D., Keen, K.L., Baumann, D.I., Filardo, E.J., and Terasawa, E. 2009.Involvement of G-protein coupled receptor 30 (GPR30) in rapid action of estrogen in primate LHRH neurons. Mol. Endocrinol. 23: 349-359. PMCID: PMC2654512
Abe, H., and E. Terasawa. 2005. Firing pattern and rapid modulation of activity by estrogen in primate LHRH-1 neurons. Endocrinology 146: 4312-4320.
Terasawa, E. and D.L. Fernandez. 2001. Neurobiological mechanisms of the onset of puberty in primates. Endocrinol. Rev. 22: 111-151. PMID: 11159818
Terasawa, E. 2001. Luteinizing-hormone releasing hormone (LHRH) neurons: Mechanism of pulsatile LHRH release. Vitam. Horm. 63: 91-129. PMID: 11358119
Terasawa, E., W.K. Schanhofer, K.L. Keen, and L.L. Luchansky. 1999. Intracellular Ca2+ oscillation in luteinizing hormone-releasing hormone (LHRH) cells derived from the embryonic olfactory placode of the rhesus monkey. J. Neurosci. 19: 5898-5909. PMID: 10407029
Terasawa, E., K.L. Keen, K. Mogi, and P. Claude. 1999. Pulsatile release of luteinizing hormone-releasing hormone in cultured LHRH neurons derived from the embryonic olfactory placode of the rhesus monkey. Endocrinology 140: 1432-1441.PMID: 10067872
Keen, K.L., Burich, A.J., Mitsushima, D., Kasuya, E., and Terasawa, E. 1999. Effects of pulsatile infusion of the GABAA receptor blocker bicuculline on the onset of puberty in female rhesus monkeys. Endocrinology 140: 5257-5266. PMID: 10537156