Professor, Department of Neuroscience
Ph.D. Massachusetts Institute of Technolog
Growth and Guidance of Axons in the Mammalian Central Nervous System
Growing axons are tipped by motile growth cones that interact with guidance cues to find appropriate targets. In some CNS pathways axon branches rather than the primary growth cone extend into and innervate targets. We are using cultured neurons from the developing cortex and time lapse fluorescence digital imaging to determine mechanisms of axon branching. We are investigating how the cytoskeleton reorganizes during axon branching by fluorescently labeling actin filaments and microtubules with microinjection techniques and visualizing dynamic cytoskeletal movements in living cortical neurons. To determine how guidance cues influence the direction of axon growth, we are applying such molecules to cortical growth cones and visualizing their effects on the cytoskeleton. We are also imaging calcium oscillations to test their role in cortical axon branching and exploring the role of actin regulatory proteins in modulating rates of growth cone advance leading to development of axon branches.
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