Mark S. Brownfield

Mark Brownfield
Title
Associate Professor, Department of Comparative Biosciences, School of Veterinary Medicine
Phone
(608) 263-5863
E-mail
brownm@svm.vetmed.wisc.edu

Education:

Ph.D. Colorado State University

Research Focus:

Role of Brain Serotonin Neurons on Neuroendocrine and Automatic Systems

Research Strengths:

Molecular Neuroscience; Neural Circuits

Research Description:

Research in my laboratory seeks to understand the structural organization of the brain serotonergic system and its impact on brain function, particularly regulation of the neuroendocrine and autonomic systems. Our approaches include light and electron microscopic immunohistochemistry, neurochemistry, neuropharmacology, and whole animal physiology.

Most recently we have focused on the molecular anatomy of serotonergic neurons and their target neurons. To support this activity we have developed a panel of antibodies against the serotonergic system. These include antisera to serotonin (5-hydroxytryptamine), its precursor (5-hydroxytryptophan), and primary metabolite (5-hydroxyindole acetic acid). Using computer-assisted antigen design we have also generated antibodies to tryptophan hydroxylase (the rate limiting enzyme in the biosynthesis of serotonin), serotonin transporter (the target of a class of antidepressant drugs), and five serotonin receptors (5HT1a, 5HT2a, 5HT2c, 5HT5a, and 5HT7). Using this unique panel of reagents we are now performing chemical mapping of 5HT neurons relative to their receptor bearing neural targets and to other neurotransmitter systems to gain information about the functional activities of the serotonergic system. These studies reveal associations with circuits important in neuroendocrine, autonomic, emotional, circadian, visual, and motor systems.

Publications:

Please see PubMed for most recent publications