Xinzhi (Richard) Chen

Research Project

My major project is to investigate relationships of changes in ion homeostasis and endoplasmic reticulum (ER) stress in response to ischemic neuronal damage. Disruption of Na+ and Ca2+ homeostasis plays a role in neuronal damage. I have found that inhibition of Na-dependent chloride cotransport is neuroprotective following in vitro ischemia. Currently, I study whether blocking Ca2+ dysregulation would reduce ER stress. I use immunoblot, immunocytochemical and SiRNA techniques to detect ER stress against several ER stress protein antibodies.

Abstracts and Publications:

Wang Y., J. Luo, X. Chen, H. Chen, S. W. Cramer, and D. Sun. 2008. Gene inactivation of Na+/H+ exchanger isoform 1 attenuates apoptosis and mitochondrial damage following transient focal cerebral ischemia. Eur. J. Neurosci. [PDF]
Chen, X. and D. Sun. 2008. ER Ca2+ dysregulation and ER stress in cerebral ischemic damage. Front. Biosci. [PDF]
Luo J., Y. Wang, H. Chen, D.B. Kintner, S.W. Cramer, J.K. Gerdts, X. Chen, G.E. Shull, K.D. Philipson, and D. Sun. 2008. A concerted role of Na+-K+-Cl- cotransporter and Na+/Ca2+ exchanger in ischemic damage. J. Cereb. Blood Flow Metab. 28(4):737-46.
Kintner D.B., X. Chen, S.W. Cramer, J. Luo, and D. Sun. 2007. Dysregulation of endoplasmic reticulum calcium in response to oxygen glucose deprivation and reoxygenation: neurons vs. astrocytes. Soc. Neurosci. Abstr.
Chen, X. and D. Sun. 2007. Na+-K+-Cl- cotransporter contributes to ER stress in neurons following in vitro ischemia. Soc. Neurosci. Abstr.
Luo, J., Y. Wang, X. Chen, H. Chen, D.B. Kinter, G.E. Shull, K.D. Phillips, and D. Sun. 2007. Increased tolerance to ischemic neuronal damage by knockdown of Na+-Ca+ exchanger isoform1. Ann. N.Y. Acad. Sci. 1099: 292-305.
Adibhatla, R.M., J.F. Hatcher, E.C. Larsen, X. Chen, D. Sun, and F.H. Tsao. 2006. CDP-choline significantly restores the phosphatidylcholine levels by differentially affecting phospholipase A2 and CTP-phosphocholine cytidylyltransferase after stroke. J. Biol. Chem. 281: 6718-6725. [PDF]
Chen, X., J. Luo, D.B. Kintner, Y. Wang, and D. Sun. 2006. Na-K-Cl cotransporter contributes to neuronal ER stress andmitchondrial dysfunction following ischemia. Soc. Neurosci. Abstr.
Chen, X., Y. Wang, H. Chen, D.B. Kintner, K.D. Philipson, and D. Sun. 2006. Knockdown of Na+/Ca2+ exchanger isoform 1 (NCX1) with SiRNA is neuroprotective in cerebral focal ischemia. Neurosci. Abstr.


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Xinzhi (Richard) Chen E-mail: x.chen@neurosurg.wisc.edu Research Project My major project is to investigate relationships of changes in ion homeostasis and endoplasmic reticulum (ER) stress in response to ischemic neuronal damage. Disruption of Na+ and Ca2+ homeostasis plays a role in neuronal damage. I have found that inhibition of Na-dependent chloride cotransport is neuroprotective following in vitro ischemia. Currently, I study whether blocking Ca2+ dysregulation would reduce ER stress. I use immunoblot, immunocytochemical and SiRNA techniques to detect ER stress against several ER stress protein antibodies. Abstracts and Publications: Wang Y., J. Luo, X. Chen, H. Chen, S. W. Cramer, and D. Sun. 2008. Gene inactivation of Na+/H+ exchanger isoform 1 attenuates apoptosis and mitochondrial damage following transient focal cerebral ischemia. Eur. J. Neurosci. [PDF] Chen, X. and D. Sun. 2008. ER Ca2+ dysregulation and ER stress in cerebral ischemic damage. Front. Biosci. [PDF] Luo J., Y. Wang, H. Chen, D.B. Kintner, S.W. Cramer, J.K. Gerdts, X. Chen, G.E. Shull, K.D. Philipson, and D. Sun. 2008. A concerted role of Na+-K+-Cl- cotransporter and Na+/Ca2+ exchanger in ischemic damage. J. Cereb. Blood Flow Metab. 28(4):737-46. Kintner D.B., X. Chen, S.W. Cramer, J. Luo, and D. Sun. 2007. Dysregulation of endoplasmic reticulum calcium in response to oxygen glucose deprivation and reoxygenation: neurons vs. astrocytes. Soc. Neurosci. Abstr. Chen, X. and D. Sun. 2007. Na+-K+-Cl- cotransporter contributes to ER stress in neurons following in vitro ischemia. Soc. Neurosci. Abstr. Luo, J., Y. Wang, X. Chen, H. Chen, D.B. Kinter, G.E. Shull, K.D. Phillips, and D. Sun. 2007. Increased tolerance to ischemic neuronal damage by knockdown of Na+-Ca+ exchanger isoform1. Ann. N.Y. Acad. Sci. 1099: 292-305. Adibhatla, R.M., J.F. Hatcher, E.C. Larsen, X. Chen, D. Sun, and F.H. Tsao. 2006. CDP-choline significantly restores the phosphatidylcholine levels by differentially affecting phospholipase A2 and CTP-phosphocholine cytidylyltransferase after stroke. J. Biol. Chem. 281: 6718-6725. [PDF] Chen, X., J. Luo, D.B. Kintner, Y. Wang, and D. Sun. 2006. Na-K-Cl cotransporter contributes to neuronal ER stress andmitchondrial dysfunction following ischemia. Soc. Neurosci. Abstr. Chen, X., Y. Wang, H. Chen, D.B. Kintner, K.D. Philipson, and D. Sun. 2006. Knockdown of Na+/Ca2+ exchanger isoform 1 (NCX1) with SiRNA is neuroprotective in cerebral focal ischemia. Neurosci. Abstr.

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