Synaptic Physiology of Epileptiform Activity
Office Phone: (608) 261-1470, (608) 263-5448
The main goal of my laboratory is to understand the synaptic physiology of the hippocampus and how that circuitry favors epileptiform activity. We use the hippocampal slice preparation and intra- and extracellular recording techniques to study synaptic potentials and currents in CA3 pyramidal neurons. The hippocampal slice preparation is capable of generating epileptiform activity when convulsants are applied acutely or following lesions that alter the intrinsic synaptic circuitry.
The lab is now investigating the effects of activation of the metabotropic glutamate receptors (mGluR) on epileptiform activity and the development of a long lasting change in physiology that promotes abnormal synchronization. Group I agonists not only produce recurrent, synchronous neuronal oscillations, but following a 2 hour exposure induce a state of long-lasting changes in synaptic circuitry that favors synchronization. We believe that this phenomenon may contribute to the epileptogenesis, the development of epilepsy.
- Karr, L and Rutecki, P.A. Activity-dependent induction and maintenance of epileptiform activity produced by group I metabotropic glutamate receptors in the rat hippocampal slice. Epilepsy Res. 81: 14-23, 2008. PMID: 18495430
- Pan, Y-Z, Karr, L, Rutecki P. Ictal activity induced by group I metabotropic glutamate receptor activation and loss of afterhyperpolarizations. Neuropharmacology, In press.
- Karr, L, Pan, Y-Z, Rutecki, P. CB1 recepter antagonism prevents induction of epileptiform activity by group I metabotropic glutamate receptor activation. Epilepsia, 51 (Suppl 3):121-125, 2010.
- Hermann, B.P., M. Seidenberg, C. Dow, P. Rutecki, and A. Bhattacharya. 2006. Cognitive prognosis in chronic temporal lobe epilepsy. Ann. Neurology 60: 80-87
- Seidenberg, M., K.E. Kelly, J. Parrish, E. Geary, C. Dow, P. Rutecki, and B. Hermann. 2005. Ipsilateral and contralateral MRI volumetric abnormalities in chronic unilateral temporal lobe epilepsy and their clinical correlates. Epilepsia 46: 420-430
- Hermann, B., M. Seidenberg, L. Sears, R. Hansen, K. Bayless, P. Rutecki, and C. Dow. 2004. Cerebellar atrophy in temporal lobe epilepsy affects procedural memory. Neurology 63: 2129-2131
- Sayin, Ü. and P.A. Rutecki. 2003. Metabotropic glutamate receptor activation alters hippocampal network excitability and produces prolonged epileptiform neuronal synchronization. Epilepsy Res. 53: 186-195.
- Hermann, B., M. Seidenberg, B. Bell, P. Rutecki, R.D. Sheth, G. Wendt, D. O'Leary, and V. Magnotta. 2003. Extratemporal quantitiative MRI volumetrics and neuropsychological status in temporal lobe epilepsy. J. Int., Neuropsych. Soc. 9: 353-362
- Sayin, Ü., S. Osting, J. Hagen, P.A. Rutecki, and T. Sutula. 2003. Spontaneous seizures and loss of axo-axonic and axo-aomatic inhibition induced by repeated brief seizures in kindled rats. J. Neurosci. 23: 2759-2768.
- Hadar, E.J., Y. Yang, Ü. Sayin, and P.A. Rutecki. 2002. Suppression of pilocarpine-induced ictal oscillations in the hippocampal slice. Epilepsy Res. 49: 61-71.